Journal: Annals of Neurosciences

Article Title: 17β-Estradiol Concentration and Direct β 2 -Adrenoceptor Inhibition Determine Estrogen-Mediated Reversal of Adrenergic Immunosuppression

doi: 10.1177/09727531211070541

Figure Lengend Snippet: In silico model of adrenergic [1A] and estrogen [1B] signalling cascades in lymphocytes: The cross-talk between the adrenergic signals (1A) and estrogen-mediated signals (1B) result in specific immunomodulatory effects depending upon the estrogen concentration. The layout of the signalling pathway is elucidated from receptors to transduction into the cytosol and nuclear translocation of signalling molecules leading to specific outcomes (1C).

Article Snippet: "In silico modeling and simulation of neuroendocrine-immune modulation through adrenergic and E2 receptors in lymphocytes show differential activation of cyclic adenosine monophosphate (cAMP)," Cold Spring Harbor Laboratory, 2020.

Techniques: In Silico, Concentration Assay, Transduction, Translocation Assay

Journal: Annals of Neurosciences

Article Title: 17β-Estradiol Concentration and Direct β 2 -Adrenoceptor Inhibition Determine Estrogen-Mediated Reversal of Adrenergic Immunosuppression

doi: 10.1177/09727531211070541

Figure Lengend Snippet: Expression of molecular markers p-ERK, p-CREB, cAMP and p-Akt by lymphocytes treated with estrogen, adrenergic agonists and in combination: Simulation of lymphocytes treated with 10-6 M Estrogen for 24 hours (2A). Simulation of lymphocytes treated with terbutaline (10-6 M), phenylephrine (10-6 M) and clonidine (10-6 M) for 24 hours (2B). Simulation of lymphocytes treated with terbutaline (10-6 M), phenylephrine (10-6 M) and clonidine (10-6 M) in the presence of estrogen (10-6 M) for 24 hours (2C).

Article Snippet: "In silico modeling and simulation of neuroendocrine-immune modulation through adrenergic and E2 receptors in lymphocytes show differential activation of cyclic adenosine monophosphate (cAMP)," Cold Spring Harbor Laboratory, 2020.

Techniques: Expressing

Journal: Annals of Neurosciences

Article Title: 17β-Estradiol Concentration and Direct β 2 -Adrenoceptor Inhibition Determine Estrogen-Mediated Reversal of Adrenergic Immunosuppression

doi: 10.1177/09727531211070541

Figure Lengend Snippet: Expression of cytokines (IL-2 and IFN-g) by lymphocytes treated with estrogen, adrenergic agonists and in combination: Simulation of lymphocytes treated with 10-6 M Estrogen for 24 hours (3A). Simulation of lymphocytes treated with terbutaline (10-6 M), phenylephrine (10-6 M) and clonidine (10-6 M) for 24 hours (3B). Simulation of lymphocytes treated with terbutaline (10-6 M), phenylephrine (10-6 M) and clonidine (10-6 M) in the presence of estrogen (10-6 M) for 24 hours (3C).

Article Snippet: "In silico modeling and simulation of neuroendocrine-immune modulation through adrenergic and E2 receptors in lymphocytes show differential activation of cyclic adenosine monophosphate (cAMP)," Cold Spring Harbor Laboratory, 2020.

Techniques: Expressing

Journal: Annals of Neurosciences

Article Title: 17β-Estradiol Concentration and Direct β 2 -Adrenoceptor Inhibition Determine Estrogen-Mediated Reversal of Adrenergic Immunosuppression

doi: 10.1177/09727531211070541

Figure Lengend Snippet: Expression of antioxidant enzymes (CAT and SOD), superoxides and peroxynitrites by lymphocytes treated with estrogen, adrenergic agonists and in combination: Simulation of lymphocytes treated with 10-6 M Estrogen for 24 hours (4A). Simulation of lymphocytes treated with terbutaline (10-6 M), phenylephrine (10-6 M) and clonidine (10-6 M) for 24 hours (4B). Simulation of lymphocytes treated with terbutaline (10-6 M), phenylephrine (10-6 M) and clonidine (10-6 M) in the presence of estrogen (10-6 M) for 24 hours (4C).

Article Snippet: "In silico modeling and simulation of neuroendocrine-immune modulation through adrenergic and E2 receptors in lymphocytes show differential activation of cyclic adenosine monophosphate (cAMP)," Cold Spring Harbor Laboratory, 2020.

Techniques: Expressing

Journal: Annals of Neurosciences

Article Title: 17β-Estradiol Concentration and Direct β 2 -Adrenoceptor Inhibition Determine Estrogen-Mediated Reversal of Adrenergic Immunosuppression

doi: 10.1177/09727531211070541

Figure Lengend Snippet: Expression of survival/apoptosis signals by lymphocytes treated with estrogen, adrenergic agonists and in combination: Simulation of lymphocytes treated with 10-6 M Estrogen for 24 hours (5A). Simulation of lymphocytes treated with terbutaline (10-6 M), phenylephrine (10-6 M) and clonidine (10-6 M) for 24 hours (5B). Simulation of lymphocytes treated with terbutaline (10-6 M), phenylephrine (10-6 M) and clonidine (10-6 M) in the presence of estrogen (10-6 M) for 24 hours (5C).

Article Snippet: "In silico modeling and simulation of neuroendocrine-immune modulation through adrenergic and E2 receptors in lymphocytes show differential activation of cyclic adenosine monophosphate (cAMP)," Cold Spring Harbor Laboratory, 2020.

Techniques: Expressing

Journal: Annals of Neurosciences

Article Title: 17β-Estradiol Concentration and Direct β 2 -Adrenoceptor Inhibition Determine Estrogen-Mediated Reversal of Adrenergic Immunosuppression

doi: 10.1177/09727531211070541

Figure Lengend Snippet: Effects of increasing doses of estrogen on survival/apoptosis signals by terbutaline, phenylephrine and clonidine: Simulation of lymphocytes treated with terbutaline (10-6 M), phenylephrine (10-6 M) and clonidine (10-6 M) in the presence of increasing doses of estrogen (10-6 M (6A), 10-5 M (6B), 10-4 M (6C), 10-3 M (6D)) for 72 hours.

Article Snippet: "In silico modeling and simulation of neuroendocrine-immune modulation through adrenergic and E2 receptors in lymphocytes show differential activation of cyclic adenosine monophosphate (cAMP)," Cold Spring Harbor Laboratory, 2020.

Techniques: